| Zentrifuge 13300 g |
Yes |
Needed |
Centrifuge |
TBD |
|
|
|
Dry run sequencing, Sequencing |
|
| Eppendorf Research Plus 0,5-10 µL variable Mechanical Pipette |
Yes |
Optional |
Pipette |
https://www.eppendorf.com/de-de/eShop-Produkte/Pipettieren/Manuelles-Pipettieren-Dispensieren/Eppendorf-Research-plus-p-3123000047 |
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Agilent SureCycler 8800 PCR Thermal Cycler |
Yes |
Needed |
Thermal Cycler |
https://www.agilent.com/cs/library/usermanuals/public/G8800-93000_SureCycler%208800_German_web.pdf |
|
|
|
PCR |
|
| Quiagen TissueLyser II |
Yes |
Needed |
Cell Disruptor |
https://www.qiagen.com/us/resources/resourcedetail?id=01db8e6a-7ad3-4b50-be75-a80f5f45d028 |
|
|
|
Extraction |
|
| Spectrophotometer 260 & 280 nm for dna quantification (Eppendorf Biophotometer) |
Yes |
Needed |
DNA quantification |
https://www.marshallscientific.com/v/vspfiles/specs/BP%20manual.pdf |
Eppendorf |
|
1 |
Dry run sequencing, Extraction, Sequencing |
|
| GELATO electrophoresis and visualization system |
Yes |
Needed |
Electrophorese |
https://www.minipcr.com/wp-content/uploads/4AA94-rev-0-GELATO-Users-guide.pdf |
|
|
|
PCR |
|
| Oxford Nanopore Technologies Flow Cell Wash Kit EXP-WSH004 |
Yes |
Needed |
Wash kit |
https://store.nanoporetech.com/flow-cell-wash-kit-r9.html |
|
|
|
Dry run sequencing, Sequencing |
|
| Oxford Nanopore Technologies Ligation Sequencing Kit SQK-LSK110 |
Yes |
Needed |
Ligation kit |
https://store.nanoporetech.com/eu/ligation-sequencing-kit110.html |
|
|
|
Dry run sequencing, Sequencing |
|
| Oxford Nanopore Technologies Native Barcoding Kit SQK-NBD112.24 |
Yes |
Needed |
Barcoding kit |
https://store.nanoporetech.com/native-barcoding-kit-24.html |
|
|
|
Sequencing |
|
| Oxford Nanopore Technologies Control Expansion Kit EXP-CTL001 |
Yes |
Needed |
Expansion kit |
https://store.nanoporetech.com/control-expansion.html |
|
|
|
Dry run sequencing |
|
| Oxford Nanopore Technologies Flongle Adapter ADP-FLG001 |
Yes |
Needed |
Flongel adapter |
https://community.nanoporetech.com/requirements_documents/flongle-spec.pdf |
|
|
|
Sequencing |
|
| Oxford Nanopore Technologies Flongle Flow Cell |
Yes |
Needed |
Flongel Flow Cell |
https://store.nanoporetech.com/eu/flongle-flow-cell-pack.html |
|
|
12 |
Sequencing |
|
| Oxford Nanopore Technologies MinION Flow Cell |
Yes |
Needed |
MinION Flow Cell |
https://store.nanoporetech.com/eu/flow-cell-r10-4.html |
|
|
2 |
Sequencing |
|
| Cardboard boxes with inlays to freeze samples |
Yes |
Needed |
Freezer boxes |
|
|
|
|
Sequencing |
|
| Oxford Nanopore Technologies MinION Mk1B MIN-101B |
Yes |
Needed |
MinION Sequencer |
https://nanoporetech.com/products/minion |
|
|
2 |
Dry run sequencing, Sequencing |
|
| Eppendorf Research Plus 10-100-200 µL variable Mechanical Pipette |
Yes |
Needed |
Pipette |
https://www.eppendorf.com/de-de/eShop-Produkte/Pipettieren/Manuelles-Pipettieren-Dispensieren/Eppendorf-Research-plus-p-3123000047 |
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Eppendorf Research Plus 100-1000 µL variable Mechanical Pipette |
Yes |
Needed |
Pipette |
https://www.eppendorf.com/de-de/eShop-Produkte/Pipettieren/Manuelles-Pipettieren-Dispensieren/Eppendorf-Research-plus-p-3123000047 |
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Styropor ice box |
Yes |
Needed |
Ice box |
|
|
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Pipetting tips 100-1000 µL (blue) (to be autoclaved) |
Yes |
Needed |
Tipps |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Pipetting tips 10-200 µL (yellow) (to be autoclaved) |
Yes |
Needed |
Tipps |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Eppis 1,5 ml (normal binding) |
Yes |
Needed |
Eppis |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
Test run |
| Master mix for PCR |
Yes |
Optional |
Master mix PCR |
|
Sponsoring (expensive) ? |
50 |
|
PCR |
|
| DNA staining - Midori green Direct |
Yes |
Optional |
DNA staining |
|
Sponsoring (expensive) ? |
|
|
PCR |
|
| Eppis 200 µl (normal binding) |
Yes |
Needed |
Eppis |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
Test run |
| Nuclease free water (to be autoclaved) |
Yes |
Needed |
Water |
|
Self made ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| White, yellow, blue tip boxes |
Could be more |
Needed |
Tipp boxes |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Tubes big (50ml) |
Yes |
TBD |
Tubes |
|
Eppendorf ? |
|
|
Extraction |
|
| 1 terrabyte SSD |
Yes |
TBD |
SSD |
|
|
|
|
Data analysis |
|
| 16 ram |
Yes |
TBD |
Ram |
|
|
|
|
Data analysis |
|
| Refrigerated Centrifuge |
No |
TBD |
Refrigerated centrifuge |
|
|
|
|
Sequencing |
|
| QIAGEN DNeasy PowerMax Soil Kit-MO BIO PowerMax Soil DNA Isolation Kit |
No |
TBD |
DNA extraction kit |
|
Sponsoring (expensive) ? |
|
|
Extraction |
|
| Zymoresearch - Quick-DNA Fecal-Soil Microbe Kit |
No |
TBD |
DNA extraction kit |
|
Sponsoring (expensive) ? |
|
|
Extraction |
|
| Centrifuge adapter for 0.2 ml eppis |
No |
Optional |
Centrifuge adapter |
https://www.btlabsystems.com/0.2ml_rotor_adapters |
|
|
|
Dry run sequencing, Sequencing |
|
| Hula mixer (gentle rotator mixer) |
No |
Needed |
Mixer |
|
Self made ? |
|
|
Dry run sequencing, Sequencing |
|
| Eppis 1,5 ml (low DNA binding) |
No |
Needed |
Eppis |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
Test run |
| Eppi rack |
No |
Needed |
Racks |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Tubes small (15ml) |
No |
TBD |
Tubes |
|
Eppendorf ? |
|
|
Extraction |
|
| Cell strainer 70 µl |
No |
TBD |
Cell strainer |
|
Eppendorf ? |
|
|
Extraction |
|
| Eppis 200 µl (low DNA binding) |
No |
Needed |
Eppis |
|
Eppendorf ? |
|
|
Dry run sequencing, Extraction, Sequencing |
Test run |
| Cell strainer 30 µl |
No |
TBD |
Cell strainer |
|
Eppendorf ? |
|
|
Extraction |
|
| Crushed Ice |
No |
Needed |
Ice |
|
Supermarket |
|
2 |
Dry run sequencing, Extraction, Sequencing |
|
| Magnet for eppi rack |
No |
TBD |
Magnet |
|
Self made ? |
|
|
Sequencing |
|
| 90% — 70% alcohol |
No |
Needed |
Alcohol |
|
Self made ? |
|
|
Dry run sequencing, Extraction, Sequencing |
|
| Nap next companion module cat E7180S or E7180L or M6630 or E7546 or E6056 |
No |
Needed |
Companion module |
|
Sponsoring (expensive) ? |
|
|
Sequencing |
|
| Magnetic beads |
No |
TBD |
Magnetic beads |
|
Sponsoring (expensive) ? |
|
|
Sequencing |
|
| Force 712 Micro-Centrifuge C9414-230V |
Yes |
Optional |
Centrifuge |
https://www.alys-technologies.com/ALYS_Labware/LN/ALYS_Labware_LN_Force_family_2002.pdf |
|
|
|
Dry run sequencing, Sequencing |
|